Orthopedic implants, prostheses, and open fractures are highly susceptible to infections and biofilm formation. These burdens require prompt prophylaxis and/or therapeutic approaches. To this end, we employ preclinical models of infection (e.g. femur fracture, intra-canal implantation) to test antimicrobial strategies for treatment and prevention of biofilm implant-related infections,.  by investigating new therapeutic or prophylaxis strategies, evaluating the host response to pathogens, and characterizing organisms related to implant infections. We employ imaging techniques, including micro-computed tomography, scanning electron microscopy, and confocal microscopy, to evaluate the biofilm deposition on tissues and orthopedic implants.