Microfluidics exploits a multidisciplinary approach to design systems where micro-volumes of fluids can be precisely controlled and manipulated. The use of microfluidic systems coupled with real-time high resolution imaging has revolutionized the field of cell biology providing the opportunity to investigate cell behavior within in vivo-like microenvironments. We design and manufacture microfluidic devices for a variety of research applications. Specifically, we developed an easy-to-use platform embedding flexible PDMS electrodes and carbon nanotubes to generate uniform electrical fields for cell stimulation. We fabricated magnetic reversibly sealable microfluidic devices by implementing PDMS/iron micropowder layers. Also, we exploited microfluidic devices to investigate the role of fluid dynamics on cell alignment, cell docking, and cell spheroid generation by coupling different experimental data with computational models.